Chymotryptic digestion greatly weakened the opiate activities of ОІh-endorphin, whereas carboxypeptidase A, tryptic and leucine aminopeptidase digests showed only small losses in potency. Anisylation of the C-terminal glutamic acid residue of ОІh-endorphin produced only small reductions in activity. Derivatives of ОІ-endorphins altered within the amino-terminal five residues showed pronounced losses in activity. Amino acid analysis and assay of the leucine aminopeptidase digests suggest that the larger potency of ОІ-endorphin relative to Met-enkephalin may be a consequence of its greater resistance to exopeptidase attack.ДЄB - The opiate activities of some derivatives and enzymatic digests of camel and human ОІ-endorphin were determined in the guinea pig ileum and rat brain opiate receptor binding assays. The C-terminus of ОІ-endorphin appears to contribute little directly to opiate activity. N2 - The opiate activities of some derivatives and enzymatic digests of camel and human ОІ-endorphin were determined in the guinea pig ileum and rat brain opiate receptor binding assays. 1973 Jun 19 52(4):1314вЂ“1319.T2 - Structure-activity relationships in the guinea pig ileum and opiate receptor binding assays Inhibition of the release of growth hormone in vitro by -melanocyte stimulating hormone.

Schally AV, Arimura A, Kastin AJ, Uehara T, Coy DH, Coy EJ, Takahara J.

Isolation of methionine sulfoxide analogue of alpha-melanocyte-stimulating hormone from bovine pituitary glands. The amino acid sequence of a peptide with growth hormone-releasing activity isolated from porcine hypothalamus.

Schally AV, Baba Y, Nair RM, Bennett CD.

Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33-46 of the alpha-chain of porcine hemoglobin.

Schally AV, Huang WY, Redding TW, Arimura A, Coy DH, Chihara K, Chang RC, Raymond V, Labrie F.

Regional dissociation of beta-endorphin and enkephalin contents in rat brain and pituitary.

Rossier J, Vargo TM, Minick S, Ling N, Bloom FE, Guillemin R.Neurons containing beta-endorphin in rat brain exist separately from those containing enkephalin: immunocytochemical studies. Bloom F, Battenberg E, Rossier J, Ling N, Guillemin R.

The opiate receptor and morphine-like peptides in the brain. Opioid peptide enkephalin: immunohistochemical mapping in rat central nervous system.

Simantov R, Kuhar MJ, Uhl GR, Snyder SH.

gamma endorphin, alpha endorphin and Met-enkephalin are formed extracellularly from lipotropin C fragment. Activity of VIP, somatostatin and other peptides in the mouse vas deferens assay.

Kastin AJ, Coy DH, Schally AV, Meyers CA.

Synthesis and opioid activities of stereoisomers and other D-amino acid analogs of methionine-enkephalin.

Coy DH, Kastin AJ, Schally AV, Morin O, Caron NG, Labrie F, Walker JM, Fertel R, Berntson GG, Sandman CA.

Strategy and tactics in protein chemistry. Isolation of the luteinizing hormone and follicle-stimulating hormone-releasing hormone from porcine hypothalami.

Schally AV, Nair RM, Redding TW, Arimura A.

Isolation and structure of somatostatin from porcine hypothalami.

Schally AV, Dupont A, Arimura A, Redding TW, Nishi N, Linthicum GL, Schlesinger DH.

alpha-Neo-endorphin : a 'big' Leu-enkephalin with potent opiate activity from porcine hypothalami.

Isolation, primary structure, and synthesis of alpha-endorphin and gamma-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity. C fragment of lipotropin has a high affinity for brain opiate receptors. The synthesis and opiate activity of beta-endorphin. Li CH, Lemaire S, Yamashiro D, Doneen BA.Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands. Opioid activity of a peptide, beta-lipotropin-(61-91), derived from beta-lipotropin. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Hughes J, Smith TW, Kosterlitz HW, Fothergill LA, Morgan BA, Morris HR.

Endorphin structure